Fig 1: STC1 is expressed at the basal layer of epidermis. (a) Immunofluorescence and (b) immunohistochemical staining of STC1 in a representative acetone-fixed specimen of abdominal skin or skin from the inner side of the upper arm of a female subject. Nuclei were stained with DAPI or hematoxylin, respectively
Fig 2: STC1 negatively influences dermal fibroblasts though the JNK pathway. mRNA abundance of (a) MMP1, (b) collagen1A1, (c) elastin in dermal fibroblasts treated with 5 µM JNK inhibitor (SP600125) for 3 hr, followed by treatment with 10 µg/mL STC1 for 24 hr. The negative influence of STC1 on mRNA expression in dermal fibroblasts (STC1) was significantly reversed by SP600125 (STC + SP), whereas SP600125 alone (SP) had no effect. mRNA abundance was expressed as mean ± SEM of three wells in each group. The statistical significance of differences between groups was determined by Dunnett's test. *: p < .05, ***: p < .001 of (STC + SP) compared with (STC1). (d) STC1 induces AP-1 nuclear translocation (downstream signal of JNK). Phospho-c-JUN was immunocytochemically stained in dermal fibroblasts at the indicated time after addition of STC1. Nuclei were stained with DAPI
Fig 3: STC1 contributes to maintenance of MMP1 expression in HaCaT cells(a) Knockdown of STC1 mRNA with siRNA in HaCaT cells. mRNA abundance at 48 hr after transfection of siRNA for STC1 (STC), negative control siRNA (Nega), or no transfection (Cont) was expressed as mean ± SEM of three wells in each group. (b) MMP1 mRNA was decreased in the siRNA-treated HaCaT cells. The statistical significance of differences between groups was determined by Dunnett's test. *: p < .05, ***: p < .001 compared with Nega
Fig 4: Inhibition of STC1 in HaCaT cells reduces the negative influence of HaCaT cells on matrix-related gene expression of dermal fibroblasts in the coculture model. (a) Dermal fibroblasts were cocultured with or without HaCaT cells in Transwells. After 2 days of coculture, the abundance of (b) MMP1, (c) collagen1A1, and (d) elastin mRNAs in dermal fibroblasts was negatively influenced (Cont) compared with fibroblasts alone (blank). The changes were significantly ameliorated in dermal fibroblasts cocultured with HaCaT cells transfected with siRNA for STC1 (STC) compared with dermal fibroblasts cocultured with HaCaT cells transfected with negative control siRNA (Nega). The statistical significance of differences between groups was determined by Dunnett's test. ***: p < .001 (STC) versus (Nega)
Fig 5: STC1 negatively regulates dermal fibroblast matrix-related gene expression. mRNA abundance of (a) MMP1, (b) collagen1A1, and (c) elastin in human dermal fibroblasts at 48 hr after addition of STC1, expressed as mean ± SEM of three wells in each group. The statistical significance of differences between groups was determined by Dunnett's test. n.s., Not significant; *, p < .05; **, p < .01; ***, p < .001 compared with control
Supplier Page from MilliporeSigma for Anti-STC1 antibody produced in rabbit